Journal: Biomaterials

Article Title: A novel Kupffer cell-targeting nanoparticle system to Mitigate alcohol-associated liver disease

doi: 10.1016/j.biomaterials.2025.123623

Figure Lengend Snippet: Release of DEX from NPs in 1X PBS for 21 days at 37°C and two different pH: 6.0 and 7.4; (a) PC-0.2+DEX; (b) PC-0.5+DEX; (c) PC-1+DEX: only PC-1+DEX showed significantly high pH-dependent release of DEX; n = 3 (d) cytocompatibility studies in (i) HepG2 cells and (ii) DTHP1 (macrophages) confirmed cytocompatibility of PC-1 and PC-1+INT NPs in both cell lines 24 h after treatment; n = 4.

Article Snippet: Human hepatocellular carcinoma cell line HepG2 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Biomaterials

Article Title: A novel Kupffer cell-targeting nanoparticle system to Mitigate alcohol-associated liver disease

doi: 10.1016/j.biomaterials.2025.123623

Figure Lengend Snippet: Uptake of NPs by DTHP1 and HepG2 cells treated with 1 μg/ml LPS and 0.16 % (v/v) EtOH; (a) quantitative analysis of NP uptake at different concentrations, confirmed higher, dose-dependent uptake by DTHP1 cells.; n = 4 (b) fluorescence imaging of NP uptake also confirmed higher uptake in DTHP1 than in HepG2 cells: green-coumarin-6 loaded NPs, blue-nuclei (scale bar = 150 μm) (c) quantification of NP uptake after cells were pre-treated with free INT (0.1 μM and 1 μM); uptake of the PC-1+INT NPs reduced due to the free INT pretreatment, confirming Gpbar1 targeting capabilities of the NPs.

Article Snippet: Human hepatocellular carcinoma cell line HepG2 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Fluorescence, Imaging

Journal: RSC Advances

Article Title: Novel diazenyl chalcones with a 2-arylidene hydrazineylidene thiazole moiety: synthesis, anticancer potential, spectroscopic characterisation, and molecular docking studies

doi: 10.1039/d5ra07769a

Figure Lengend Snippet: Fluorescent microscopy analysis of HepG2 cells treated with synthetic compounds (4–6a,b) and stained with acridine orange/ethidium bromide (AO/EB).

Article Snippet: Normal human skin fibroblasts (HSF), the human breast cancer epithelial cell line (MDA), and the human liver cancer cell line (HepG2) were purchased from VACCERA Egypt and obtained from the American Type Culture Collection (ATCC).

Techniques: Microscopy, Staining

Journal: RSC Advances

Article Title: Novel diazenyl chalcones with a 2-arylidene hydrazineylidene thiazole moiety: synthesis, anticancer potential, spectroscopic characterisation, and molecular docking studies

doi: 10.1039/d5ra07769a

Figure Lengend Snippet: (A) Microscopic view of the cell migration inhibition assay of the HepG2 cell line after 48 hours. (B) Quantitative analysis of the wound area by calculating the wound closure percentage and migration rate after 48 hours, compared to the initial time (0 hours). (C) Relative fold change in the gene expression of MMP-2 and MMP-9 in cells treated with synthetic compounds (4–6a,b) in HepG-2. All assays were conducted in triplicate, and the results are expressed as mean ± SEM. MMP: matrix metalloproteinase.

Article Snippet: Normal human skin fibroblasts (HSF), the human breast cancer epithelial cell line (MDA), and the human liver cancer cell line (HepG2) were purchased from VACCERA Egypt and obtained from the American Type Culture Collection (ATCC).

Techniques: Migration, Inhibition, Gene Expression

Journal: RSC Advances

Article Title: Novel diazenyl chalcones with a 2-arylidene hydrazineylidene thiazole moiety: synthesis, anticancer potential, spectroscopic characterisation, and molecular docking studies

doi: 10.1039/d5ra07769a

Figure Lengend Snippet: Structure–activity relationship (SAR) of the synthesised azo derivatives (4–6a,b) showing the effect of aromatic substitution on cytotoxic selectivity. Selectivity index (SI) values against MDA-MB-231 and HepG2 cells relative to WI-38 normal cells are shown.

Article Snippet: Normal human skin fibroblasts (HSF), the human breast cancer epithelial cell line (MDA), and the human liver cancer cell line (HepG2) were purchased from VACCERA Egypt and obtained from the American Type Culture Collection (ATCC).

Techniques: Activity Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mitochondrial and lipid metabolism rewiring during HEV infection

doi: 10.1007/s00018-025-05994-1

Figure Lengend Snippet: Proteomic analysis of HEV-1-infected cells. a , Confocal microscopy of mock- and HEV-1-infected HepG2/C3a-MAVS-KD cells stained for ORF2 protein (red) and nuclei (blue). Scale bar, 20 μm. Right: Quantification of infection rate. Each data point represents the percentage of ORF2-positive cells from 16 microscopic areas (~ 300 cells) across three independent experiments. Statistical significance was assessed using a nonparametric Student’s t-test, p < 0.0001 (****). b , Venn diagram of identified proteins (left) and volcano plot (right) showing differential protein abundance between HEV-1-infected and mock-infected cells (EdgeR). Dashed line indicates statistical significance threshold (− log₁₀ p-value); vertical lines denote significantly upregulated (magenta) and downregulated (blue) proteins. c , Heatmap with hierarchical clustering of significantly altered proteins. Data represent mean values from three independent experiments. Z-score–normalised expression is colour-coded (red, upregulation; blue, downregulation). d , Sankey plot of significantly enriched canonical pathways grouped by biological function (e.g., immune response, metabolism), as identified by Ingenuity Pathway Analysis (IPA). Each ribbon represents a pathway, colour-coded by functional category. Right, bubble plot showing pathway activation status: bubble colour indicates predicted z-score (red, activated; blue, inhibited; gray, no available pattern), with bubble size reflecting the number of overlapping proteins and x-axis showing − log₁₀ p -value. e , Chord diagram highlighting proteins contributing to two or more enriched pathways enriched pathways related to mitochondrial function, stress responses, and lipid metabolism, as well as autophagy and apoptosis. Connections indicate protein membership in the leading-edge subsets of each pathway (circlize R package). f , Network diagram showing functional interactions between enriched pathways based on co-enrichment and shared proteins. Nodes represent pathways, and edges indicate functional similarity or shared components, illustrating coordinated host cell reprogramming

Article Snippet: HepG2/C3a, a subclone of the human hepatocarcinoma cell line HepG2, was obtained from ATCC (CRL-3581) and used for viral production.

Techniques: Infection, Confocal Microscopy, Staining, Quantitative Proteomics, Expressing, Functional Assay, Activation Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mitochondrial and lipid metabolism rewiring during HEV infection

doi: 10.1007/s00018-025-05994-1

Figure Lengend Snippet: HEV infection reprograms the neutral lipid metabolism. a , HepG2/C3a-MAVS-KD cells were infected with HEV-1 or HEV-3 genotypes for three weeks, to reach at least 50%. Large field micrographs showing nuclei (blue) and ORF2 staining in infected cells (green), scale bar 50 µM (left). Bar graph showing quantification of infection rate using large field microphaphs and (right). Data points represent percentage of ORF-2 positive cells from 25 microscopic areas from three independent experiment (10 4 cells) using StarDist tool. b , Neutral lipid (NL) were extracted from mock- (black), HEV-1- (red) and HEV-3-infected (blue) HepG2/C3a-MAVS-KD cells and quantified by comparing the peak area of individual species to class-specific internal standards (left panel). Left, total NL abundance (sum of CL, CE, and TG per mg protein). Right, The three main NL classes—cholesterol (CL), cholesteryl esters (CE), and triglycerides (TG) - relative proportions of CL, CE, and TG as percentages of total NL. c - d , Relative abundance (per mg protein) of the most significantly altered cholesterol (CL-C18, c ) and triglyceride subspecies ( d ). e , Heatmap showing the relative abundance profiles of individual NL subspecies. Each column represents a biological replicate; each row corresponds to a lipid species. f , Lipid droplet (LD) content visualised by Bodipy 493 − 503 staining using confocal microscopy. g , Representative FACS histograms gated on bodipy positive cells (left). Bar graph showing mean fluorescence intensity (MFI) from six independent experiments (right). Lipid amounts were normalised to protein level All the results are presented as mean values ± SEM from at least three independent experiments. Statistical significance was assessed using ANOVA for multiple comparisons. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

Article Snippet: HepG2/C3a, a subclone of the human hepatocarcinoma cell line HepG2, was obtained from ATCC (CRL-3581) and used for viral production.

Techniques: Infection, Staining, Confocal Microscopy, Fluorescence

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mitochondrial and lipid metabolism rewiring during HEV infection

doi: 10.1007/s00018-025-05994-1

Figure Lengend Snippet: HEV infection impairs oxylipin metabolism. HepG2/C3a-MAVS-KD cells were infected with HEV-1 or HEV-3 for at least three weeks until infection rates exceeded 50%. The abundance of 20 major oxylipins in mock- (black), HEV-1- (red), and HEV-3-infected (blue) cells was quantified and normalised to protein amounts. a , Relative abundance of metabolites derived from omega-6 linoleic acid (LA) via the LOX pathways. b , Relative abundance of oxylipins derived from arachidonic acid. c , Relative abundance of bioactive metabolites derived from the omega-3 polyunsaturated eicosapentaenoic acid and docosahexaenoic acid Data represent mean values ± SEM of three independent experiments Statistical significance was assessed by repeated measures ANOVA with Tukey post hoc test. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

Article Snippet: HepG2/C3a, a subclone of the human hepatocarcinoma cell line HepG2, was obtained from ATCC (CRL-3581) and used for viral production.

Techniques: Infection, Derivative Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mitochondrial and lipid metabolism rewiring during HEV infection

doi: 10.1007/s00018-025-05994-1

Figure Lengend Snippet: HEV infection does not implicate glycolysis metabolic pathway. HepG2/C3a-MAVS-KD cells were uninfected (Mock, black) or infected with HEV-1 (red) or HEV-3 (blue) for at least three weeks until infection rates exceeded 50%. a , Extracellular acidification rate (ECAR) was measured using the XF Glycolysis Stress Test and the Seahorse XFp analyzer. Time points of glucose, Oligomycin (Oligo) and 2-Deoxy-D-Glucose (2-DG) are indicated by dotted arrow. Data are presented as mean values ± SEM from six independent experiments. b , Violin plots comparing glycolytic flux parameters: Glycolysis, Glycolytic Capacities, Glycolytic Reserve and non-glycolytic acidification rate. Data are presented as mean values ± SEM from repeated measures of six independent experiments. c , Lactate concentration quantified in culture supernatants. Data represent mean values ± SEM of at least six independent experiments Statistical significance was assessed by repeated measures ANOVA with Tukey post hoc test. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

Article Snippet: HepG2/C3a, a subclone of the human hepatocarcinoma cell line HepG2, was obtained from ATCC (CRL-3581) and used for viral production.

Techniques: Infection, Concentration Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mitochondrial and lipid metabolism rewiring during HEV infection

doi: 10.1007/s00018-025-05994-1

Figure Lengend Snippet: HEV infection increases mitochondrial function. HepG2/C3a-MAVS-KD cells were uninfected (Mock, black) or infected with HEV-1 (red) or HEV-3 (blue) for at least three weeks until infection rates exceeded 50%. a , Mitochondrial Oxygen consumption rate (OCR) measured in Mock- (black dots) and HEV-1-infected (red dots) cells. Time point of oligomycin (Oligo), FCCP and rotenone/antimycin A (Rot/Ant) are indicated by the dotted arrow. Data are presented as mean values ± SEM from six independent experiments. b , Violin plots comparing the parameters of mitochondrial function - basal respiration, ATP production, proton leak, maximal respiration, coupling efficiency, spare respiratory capacities, and non-mitochondrial respiration (non-mito O2 consumption). Data are presented as mean values ± SEM from repeated measures of six independent experiments. c , Representative confocal micrographs showing immunostaining of mitochondria (Tom22-Green), ORF2 (red) and nucleus (blue). Scale bar 20 µM. d , Mitochondria mass and polarization were analyzed using mitotracker green and TMRM, respectively. The ratio mitotracker/TMRM denoting mitochondria polarization/mitochondria mass is presented (right panel). e , Cellular ROS production measured by CellRox probe. Flow cytometry data are presented as geometric mean fluorescence intensity (MFI) Mock-infected cells (black dots), HEV-1 infected cells (red dots), HEV-3 infected cells (blue dots). d - e , Data are presented as mean values ± SEM from six independent experiments Statistical significance was assessed by repeated measures ANOVA with post hoc test p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

Article Snippet: HepG2/C3a, a subclone of the human hepatocarcinoma cell line HepG2, was obtained from ATCC (CRL-3581) and used for viral production.

Techniques: Infection, Immunostaining, Flow Cytometry, Fluorescence

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mitochondrial and lipid metabolism rewiring during HEV infection

doi: 10.1007/s00018-025-05994-1

Figure Lengend Snippet: Fatty acid oxidation rather than glycolysis is required for HEV infection. HepG2/C3a-MAVS-KD cells were pre-treated for 1 h with the indicated inhibitor before being infected with HEV-1 or HEV-3 for 48 h in presence of the same inhibitor. a , Confocal micrographs of HEV replication in the presence of DMSO vehicle (CTRL) or different inhibitors of metabolic pathways: CD36 inhibitor (CD36-Inh), Etomoxir and Oligomycin (Oligo). ORF2 (green) and nuclei (dapi, blue). Scale bar 100 µM. b , Quantification of HEV-1(red) and HEV-3 (blue) replication under different culture conditions. Data points represented percentage of control and given as mean values ± SEM from six independent experiments. p values are computed using one-way ANOVA. p < 0.01 (**), p < 0.001 (***), p < 0.0001(****). c , Left: Confocal micrographs showing HEV-1 and HRV-3 replication in complete medium (CTRL), in the presence of 2DG inhibitor of glycolysis or under glucose restriction (ΔGlu). ORF2 staining (green) and DAPI-stained nuclei (blue). Scale Bar 100 µM Right: Quantification of HEV ORF2 positive cells. Data points represented percentage of control. Data are presented as mean values ± SEM from at least six independent experiments Statistical significance was assessed by repeated measures ANOVA with post hoc test p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

Article Snippet: HepG2/C3a, a subclone of the human hepatocarcinoma cell line HepG2, was obtained from ATCC (CRL-3581) and used for viral production.

Techniques: Infection, Control, Staining